ABSTRACT
Objective:To study the effects of Ti-TiO2 nanotubes with different diameters on the proliferation,stretching and collagen secretion of fibroblasts.Methods:TiO2 nanotubes formed at 1,5,10 and 20 V potentials served as the experimental samples and polished pure titanium served as the control.Fibroblasts was cultivated on the surface of the various samples.MTT assay was used to examine the cell proliferation.The surface morphology of the cells was observed with SEM.Collagen secrection was tested by sirius red/bitter acid staining.Results:The nanotubes prepared by 1,5,10 and 20 V were with the diameter of 15,30,50 and 100 nm respectively.At day 1,3 and 5,the cell proliferation on polished pure titanium surface was more than that on the nanotubes surfaces at the same time;at day 5,cell proliferation on 100 nm nanotubes was significantly more than that on the other nanotube surfaces (P ＜ 0.01).At day 3,fibroblasts at polished pure titanium surface stretched as typical long spindle form,while with obvious pseudopodium at nanotube surfaces.The collagen secretion of fibroblasts was highest at 100 nm nanotubes (P ＜ 0.01).Conclusion:100 nm nanotubes may have minimal negative effect on fibroblast proliferation and the greatest positive effect on the collagen secretion.
ABSTRACT
BACKGROUND:Compared with the titanium with smooth surface, TiO2 nanotubes are more beneficial for the early cel adhesion. OBJECTIVE:To review the research progress of the effect of TiO2 nanotubes on peri-implant cel s. METHODS:A computer-based retrieval of CNKI, CqVip, WanFang and PubMed databases was performed for pertinent literatures published between 1990 to 2016, using the Chinese keywords of“TiO2 nanotubes or titanium dioxide nanotubes and implant”, and English keywords of“TiO2 nanotubes implant, Tio2 nanotubes cel”. RESULTS AND CONCLUSION:The main factors that TiO2 nanotubes affect the peri-implant cel behaviors include the tubular morphology, size, TiO2 crystal structure, as wel as surface chemical compositions, roughness and hydrophilicity and free energy. The influence factors of TiO2 nanotubes for cel s are not only related to the tube diameter, but also associated with cel types. Different cel types hold different diameters suitable for the cel adhesion, proliferation and differentiation on the material surface. Therefore, it is advisable to select the best nanotube size for different tissues and cel s applied in different biomedical fields.
ABSTRACT
BACKGROUND: Diseases and maternal poor habits before conception and during pregnancy have been considered as the definite risk factors for premature delivery and one of the results with better repeatability in the studies on the pathogenesis, whether parental socioeconomic status is related to the occurrence of premature birth?OBJECTIVE: To investigate the association of parental socioeconomic status with premature birth.DESIGN: A case-control study with the parents who has premature infants or normal infants as subjects.SETTING: Department of Preventive Medicine, Medical College of Qingdao University;Woman's Department of Traditional Chinese Medicine, Qingdao Municipal Haici Medicine Group; Department of Obstetric and Gynecology, Qingdao Municipal Eighth People's Hospital PARTICIPANTS: A cohort of premature infants (n=227) were recruited from Qingdao Municipal Maternity and Children Health Institute and the Eighth People's Hospital, Qingdao, premature birth were confirmed according to the diagnostic standards; the control infants (n=750) were also recruited from the same place, they were delivered at pregnancy of from 38 to 42 weeks with birth mass of 3 000-4 000 g. Two groups were matched in sexual constitution and the birthday of within the same week.METHODS: Parents of two groups were asked to fill the self-made questionnaire. Maternal obstetric factors were referring to the perinatal booklet and infant birth record; meanwhile parental socio-economic status, daily-life behaviours and sociodemographic character were also investigated. Multivariate non-conditioned logistic regression analysis was used to assess the relationship of correlative factors.MAIN OUTCOME MEASURES: Parental school education, occupation, the annual family income, as well as bad living and behavioral habits.RESULTS: According to the real state, data of 227 premature infants and 750 normal control infants were analyzed finally. Maternal delivery age of ＞ 35 years old (≤ 25 years old in normal control group), working as a teacher or unemployment (worker in control group), smoking and drinking during pregnancy, father's occupation as peasant (worker in control group), with a long smoking history, lower parental education (not lower than junior college education in control group) and family income less than 35 thousand Yuan (more than 35 thousand Yuan in control group) were proved to be the significant risk factors for premature birth (OR =1.629-15.701).CONCLUSION: Maternal employment as a teacher with father as a peasant, together with lower family income and lower parental education are connected with premature delivery.